Effect of hydrogen peroxide with different pH values on the color, translucency and laser-induced fluorescence of human dentin
10.3760/cma.j.issn.1002-0098.2013.08.002
- VernacularTitle:不同pH值过氧化氢对人牙本质颜色、透明度和激光诱导荧光的影响
- Author:
Shan-Shan LIANG
1
;
Tao JIANG
;
Yi-Ning WANG
Author Information
1. 430079,武汉大学口腔医学院口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室
- Keywords:
Tooth bleaching;
Hydrogen peroxide;
Dentin;
Spectrum analysis,Raman
- From:
Chinese Journal of Stomatology
2013;48(8):453-457
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of 30％ hydrogen peroxide(HP) with different pH values on color,translucency and laser-induced fluorescence of human dentin.Methods Sixty dentin specimens from crown of mandibular third molars were randomly assigned into four groups (n =15) and treated with acidic 30％ HP,neutral 30％ HP,alkaline 30％ HP and deionized water (control group),respectively.The bleaching process was 0.5 h × 4 times,and time points for measuring were baseline (0),0.5,1 and 2 h.A colorimeter was employed to measure the L *,a *,b * coordinates of dentin against white,black and yellow background.Then the parameters of translucency,masking effects,chroma and whiteness were calculated.The dentinal laser-induced Raman/fluorescence spectra was recorded by a Raman spectrometer and the fluorescence intensity (FI) and FI％ were calculated.Results △FI of acidic,neutral,alkaline 30％ HP at 2 h were 9960.03 ±2037.74,8502.09 ± 1413.86,8554.29 ± 1986.19.And △FI％ were 84.60 ± 3.43,84.89 ± 5.19,86.72 ± 2.65,respectively.Repeated measure of ANOVA revealed that all parameters in the bleaching groups were significantly influenced by time (P ＜ 0.001).Compared with control group,bleaching resulted significant change of △TP,△chroma,△ whiteness,△L*,△a*,△b*,△E,△FI and △FI％ (P ＜0.001).There was no significant difference between three bleaching groups on △TP,△masking effects,△chroma,△whiteness,△L*,△b*,△E,△FI and △FI％.Correlation analysis demonstrated that FI was associated with chroma,a*,b* and whiteness,respectively,and △FI was associated with △TP,△masking effects,△whiteness,△chroma,△b* and △ E.Conclusions 30％ HP with different pH values could result in the same change of the color,translucency and laser-induced fluorescence of human dentin.Laser-induced fluorescence was associated with dentinal color and translucency,which might be a novel way to investigate the bleaching mechanism of dentin.
