Expression of quorum-sensing related genes during Enterococcus faecalis biofilm formation
10.3760/cma.j.issn.1002-0098.2013.08.009
- VernacularTitle:粪肠球菌生物膜形成过程中群体感应相关基因的表达研究
- Author:
Zhi-Shan LU
1
;
Lei MENG
;
Zhong-Hao LIU
;
Guang-Hui REN
;
Ai-Jie SUN
;
Xiang-Mei LIU
Author Information
1. 滨州医学院附属烟台市口腔医院牙体牙髓科
- Keywords:
Enterococcus faecalis;
Biofilms;
Quorum sensing;
Gene expression
- From:
Chinese Journal of Stomatology
2013;48(8):485-489
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the expression of the quorum-sensing related genes during Enterococcus faecalis (Ef) biofilm formation.Methods Ef biofilms model was established in vitro and film formation process was observed by confocal laser scanning microscope at 6,12,24 and 48 hours respectively.Quantification of biofilms was achieved by staining with crystal violet.Real-time fluorescence quantitative PCR method was used to detect the expression of fsrB,gelE and sprE genes in the process of Ef biofilm formation.Results A lot of live and dead bacteria unevenly distributed in Ef biofilm.The quantity of biofilms increased with time within 24 hours and was 0 h:0.00 ± 0.00,6 h:1.09 ± 0.13,12 h:2.10 ±0.79,24 h:3.30 ±0.13,which was significantly different among the 4 time period(P ＜ 0.05).The quantity of biofilm at 48 h(3.51 ±0.01) increased slightly compared with 24 h(3.30 ±0.13),but did not show significant difference.Quantitative real-time PCR showed that the expression of quorumsensing related fsrB increased with time within 24 hours and was 0 h:9.98 ±0.46,6 h:23.45 ± 1.13,12 h:47.30 ±2.49,24 h:331.30 ±2.18,which was significantly different among the 4 time period(P ＜0.05).The expression of gelE was 0 h:6.54 ± 0.73,6 h:14.26 ± 1.24,12 h:37.47 ± 2.35,24 h:264.80 ± 5.10 (P ＜ 0.05).The expression of sprE was 0 h:7.72 ± 0.74,6 h:21.15 ± 0.96,12 h:49.87 ± 3.18,24 h:441.89 ± 7.74,which was significantly different among the 4 time period (P ＜ 0.05).Conclusions The fsrB,gelE and sprE genes are closely related to the biofilm formation in Ef.
