Effect of sonicated extracts of Porphyromonas gingivalis on osteogenic differentiation of mouse osteoblasts
10.3760/cma.j.issn.1002-0098.2013.07.005
- VernacularTitle:牙龈卟啉单胞菌超声提取物对小鼠成骨细胞骨向分化蛋白表达的影响
- Author:
Jian-Ying ZHANG
1
;
Shao-Jie YU
;
Yun FU
Author Information
1. 中山大学光华口腔医学院·附属口腔医院牙周科·广东省口腔医学重点实验室
- Keywords:
Porphyromonas gingivalis;
Osteoblasts;
Alkaline phosphatase;
Osteogenic differentiation
- From:
Chinese Journal of Stomatology
2013;48(7):398-402
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of sonicated extracts of Porphyromonas gingivalis (Pg) on osteogenic differentiation of mouse osteoblast cell line MC3T3-E1.Methods PgW83 was cultured under standard anaerobic conditions and extracted by sonication.Mouse osteoblast cell line MC3T3-E1 was cultured with various concentrations of the extraction(0,10,100,1000 mg/L).Western blotting was applied to investigate the expression of osteocalcin(OC),bone sialoprotein(BSP),osteopo ntin(OPN)and osteonectin(ON).The activity of alkaline phosphatase (ALP) was detected by microplate reader after 14 days.Mineralization nodule formation was measured by alizarin red staining after 21 days.Results Compared with the control group,the extracts of Pg decreased OC and ON expression in a dose-dependent manner(OC relative expression:1.000 ± 0.000,0.852 ± 0.110,0.625 ± 0.451,0.213 ± 0.053),(ON relative expression:1.000 ± 0.000,1.035 ± 0.133,0.141 ± 0.023,0.020 ± 0.003) (P ＜ 0.05).The expression of OPN was down-regulated significantly in MC3T3-E1 treated with 1000 mg/L extraction (0.572 ± 0.162) compared with control group,10 and 100 mg/L (1.000 ± 0.000,1.029 ± 0.135,1.199 ± 0.337) (P ＜ 0.05).The expression of BSP remained unchanged when the cells were cultured with or without extraction (BSP relative expression:1.000 ± 0.000,0.831 ± 0.182,0.897 ± 0.115,0.778 ± 0.235) (P ＞0.05).Meanwhile,the extracts of Pg decreased ALP activity [control group:(0.0275 ±0.0014) U/gprot,10 mg/L:(0.0140 ±0.0011) U/gprot,100 mg/L:(0.0057 ±0.0013) U/gprot,1000 mg/L:(0.0020 ± 0.0008) U/gprot] (P ＜ 0.05) and reduced mineralization nodule formation.Conclusions The results suggest that Pg may inhibit osteoblasts'osteogenic function by down-regulation of osteogenic differentiation related proteins.
