Detection of multidrug resistance-associated proteins MRP2, MRP3, and MRP5 mRNA expressions in hepatocarcinoma cells using SYBR real-time PCR.
- Author:
Lei ZHANG
1
,
2
;
Chi-Hua FANG
;
Ying-Fang FAN
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; metabolism; Cell Line, Tumor; Gene Expression Regulation, Neoplastic; Humans; Multidrug Resistance-Associated Proteins; metabolism; Neoplasm Proteins; metabolism; RNA, Messenger; metabolism; Reverse Transcriptase Polymerase Chain Reaction
- From: Journal of Southern Medical University 2008;28(2):219-221
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the differential expression of genes encoding multidrug resistance-associated protein 2 (MRP2), MRP3 and MRP5 in human normal liver cell line L-02, hepatocarcinoma cell line BEL and its adriamycin-resistant counterpart BEL/ADM cell line.
METHODSThe total RNA was extracted from L-02, BEL and BEL/ADM cells, respectively, and the mRNAs were reversely transcribed into cDNA. The expression levels of MRP2, MRP3, MRP5 mRNAs were detected according to the standard curve using real-time fluorescence quantitative PCR.
RESULTSThe expression of MRP2 mRNA in BEL/ADM cell line was significantly higher than that in L-02 and BEL cells (P=0.000), and the latter two cells showed similar expression levels of MRP2 mRNA (P=0.468). The expressions of MRP3 and MRP5 mRNA showed statistically significant difference between the 3 cell lines (P=0.000).
CONCLUSIONMRP2 might play a role in the intrinsic drug resistance, and MRP3 and MRP5 are related to the acquired drug resistance of hepatocellular carcinoma.
