Optimization of supercritical fluid extraction of bioactive components in Ligusticum chuanxiong by orthogonal array design.
- Author:
Li-Cui HU
1
,
2
;
Xun WU
3
;
Xue-Dong YANG
3
Author Information
1. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China. hlc0614@
2. com
3. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
- Publication Type:Journal Article
- MeSH:
Benzofurans;
analysis;
isolation & purification;
Chromatography, Supercritical Fluid;
instrumentation;
methods;
Coumaric Acids;
analysis;
isolation & purification;
Drugs, Chinese Herbal;
analysis;
isolation & purification;
Ligusticum;
chemistry
- From:
China Journal of Chinese Materia Medica
2013;38(20):3484-3488
- CountryChina
- Language:Chinese
-
Abstract:
With the yields of ferulic acid, coniferylferulate, Z-ligustilide, senkyunolide A, butylidenephthalide, butylphthalide, senkyunolide I, senkyunolide H, riligustilide, levistolide A, and total pharmacologically active ingredient as evaluation indexes, the extraction of Ligusticum chuanxiong by supercritical fluid technology was investigated through an orthogonal experiment L9 (3(4)). Four factors, namely temperature, pressure, flow rate of carbon dioxide, co-solvent concentration of the supercritical fluid, were investigated and optimized. Under the optimized conditions, namely 65 degrees C of temperature, 35 MPa of pressure, 1 L x min(-1) of CO2 flow rate, 8% of co-solvent concetration, supercritical fluid extraction could achieve a better yield than the conventional reflux extraction using methanol. And the supercritical fluid extraction process was validated to be stable and reliable.
