Effect of Connexin43 on Mechanical Tension-stimulated Osteogenic Transcription Factors of Human Periodontal Ligament Fibroblasts.
- Author:
Sheng-nan LI
1
;
Hua-jing ZHANG
1
;
Bo HUO
2
;
Ding ZHANG
1
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Connexin 43; Fibroblasts; Glycyrrhetinic Acid; analogs & derivatives; Humans; Osteogenesis; Periodontal Ligament; RNA, Messenger; RNA, Small Interfering; Stress, Mechanical; Transcription Factors; Up-Regulation
- From: Acta Academiae Medicinae Sinicae 2016;38(1):22-26
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the expression and effect of Connexin43 (Cx43) on tensile tension-stimulated osteogenic transcription factors of human periodontal ligament fibroblasts (hPDLFs).
METHODSAfter hPDLFs were treated with 5% elongation tension for 1 h, 2 h, 4 h, 8 h, and 24 h, we examined the expressions of Cx43, Osterix, and RUNX2 at the mRNA level. After Cx43 expression was suppressed by siRNA or 18α-GA, the changes The mRNA in hPDLFs of Osterix and RUNX2 were observed.
RESULTSThe expressions of Cx43, Osterix, and RUNX2 mRNA in hPDLFs increased in a time-dependent fashion following tensile strain (all P<0.05), with the highest level at 5% elongation for 24 h. After Cx43 expression was blocked by two different methods, the increasing expressions of Osterix and RUNX2 were inhibited.
CONCLUSIONS5% cyclic tension upregulates Cx43 expression and promotes the expression of Osterix and RUNX2 in a time-dependent manner. Cx43 may be involved in the osteogenic response of hPDLFs to mechanical tension.
