Effect of Endometrial Stem Cell-derived Cytokine Cocktail on a Mouse Model of Myocardial Reperfusion Injury.
10.3881/j.issn.1000-503X.2016.03.002
- Author:
Zhi JIANG
1
;
Zhong-shen JIA
2
;
Yu-jia PAN
1
;
An-jie LI
1
;
Fang WEI
1
Author Information
1. Department of Cardiology,Guizhou Provincial People’s Hospital,Guizhou 550002,China
2. Department of Clinical Medicine,Guizhou Medical University,Guizhou 550025,China
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Caspase 3;
metabolism;
Cells, Cultured;
Cytokines;
therapeutic use;
Disease Models, Animal;
Endometrium;
cytology;
Female;
Mice;
Myocardial Reperfusion Injury;
therapy;
Myocytes, Cardiac;
drug effects;
Stem Cells;
cytology
- From:
Acta Academiae Medicinae Sinicae
2016;38(3):253-259
- CountryChina
- Language:English
-
Abstract:
Objective To study the effect of endometrial stem cells (EnSCs) derived cytokine cocktail (EdCC) on myocardial ischemic reperfusion injury (I/R) in a mouse model. Methods EdCC was concentrated from the culture medium of EnSCs with Millipore ultra-filtration technology and was administrated to a myocardial I/R mouse models through tail vein injection. The infarct area was determined by TTC/Evans Blue staining. The apoptotic cells were counted by TUNEL assay and the protein level of cleaved caspase 3 was evaluated by Western blotting. Results The EdCC extraction efficiency was (222.4±29.3) Μg/10(6) cells in every 24 h,but the protein gradually degraded under-80 ℃ storage. As compared with I/R group,100 Μg fresh EdCC decreased infarct area (P=0.001),reduced apoptotic nuclei in the infarct border (P=0.019),and inhibited cleaved caspase 3 expression (P=0.002). Increasing EdCC dosage did not further reduce the infarct area. The myocardial protective effect of EdCC diminished after 90 days' storage under-80 ℃. Conclusion EdCC reduces myocardial I/R injury through protecting cardiomyocytes from apoptosis within 30 days storage under-80 ℃.
