Research of the feeding strategy in the fermentation of recombinant human fibreblast growth factor mutant.
- Author:
Hui YUAN
1
;
Xiao-Kun LI
;
Shu-Lin YANG
Author Information
1. Bio-natural Pharmaceutical Institute of Wenzhou Medical College, Wenzhou 325035, China.
- Publication Type:Journal Article
- MeSH:
Acetic Acid;
metabolism;
Bioreactors;
microbiology;
Cell Culture Techniques;
methods;
Culture Media;
Escherichia coli;
genetics;
metabolism;
Fermentation;
Fibroblast Growth Factor 1;
biosynthesis;
genetics;
Humans;
Mutant Proteins;
biosynthesis;
genetics;
Recombinant Proteins;
biosynthesis;
genetics;
isolation & purification
- From:
Chinese Journal of Biotechnology
2006;22(2):322-327
- CountryChina
- Language:Chinese
-
Abstract:
Human acidic fibreblast growth factor (haFGF) was a kind of cell growth factor with wide bio-activity on cell from mesectoderm and neuro-ectoderm.In this paper, the effect of acetate concentration on the growth and expression of recombinant human acidic fibroblast growth factor mutant system E.coli BL21(DE3)/pET3C-haFGF was investigated. Four fed-batch modes: batch-fed, batch-DO static balance, DO static balance-glucose starvation, and pH-static state were investigated. The accumulation of acetate during the fermentation course was effectively inhibited. The OD600nm value was about 22, after purification, the soluble rhaFGF yielded 450mg/L. During the fermentation, no special ways such as pure oxygen, pressure were adopted, thus the established process would be easily scaled up for industry purpose.
