Effect of medium components on callus induction and contents of polysaccharides in calli from Achyranthes bidentata.
- Author:
Ming-Jun LI
1
,
2
;
Ping LI
;
Xiao-Li ZHANG
;
Xi-Ting ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Achyranthes; cytology; drug effects; growth & development; metabolism; Cell Culture Techniques; Culture Media; pharmacology; Drugs, Chinese Herbal; chemistry; Plant Growth Regulators; pharmacology; Plant Leaves; cytology; drug effects; growth & development; metabolism; Plant Stems; cytology; drug effects; growth & development; metabolism; Polysaccharides; metabolism; Tissue Culture Techniques
- From: China Journal of Chinese Materia Medica 2008;33(7):745-749
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of medium components on the callus induction and the contents of polysaccharides in calli from Achyranthes bidentata.
METHODLeaves and stems were selected as explants. The effects of six kinds of factors including basal culture medium, carbon source, 2,4-D, 6-BA, TDZ, CH on the callus induction and the contents of ABPS in calli on the high growth point were studied by orthogonal design method. The data were analyzed with range analysis and variance analysis.
RESULTTo leaf, the optimal medium of callus induction was B5 with 2 mg x L(-1) 2,4-D, 0.5 mg x L(-1) 6-BA, 30 g x L(-1) glucose and 1 g x L(-1) CH; to stem, the optimal one was B5 with2 mg x L(-1) 2,4-D, 1 mg x L(-1) 6-BA, 30 g x L(-1) glucose and 0.5 g x L(-1) CH. In order to obtain higher contents ABPS, to leaf calli, the optimal medium was LS with 1 mg x L(-1) 6-BA, 1 mg x L(-1) TDZ and 30 g x L(-1) sucrose; to stem calli, the optimal one was LS with 1 mg L(-1) 2,4-D, 0.5 mg x L(-1) 6-BA, 1 mg x L(-1) TDZ and 30 g x L(-1) glucose.
CONCLUSIONThe optimal media of callus induction were established with stems and leaves of A. bidentata as explants and with a view to an industrial production of polysaccharides by tissue and cell culture.
