Clinicopathological and prognostic significance of the expression of ADAM17 mRNA and protein in esophageal squamous cell carcinoma.
- VernacularTitle:去整合素-金属蛋白酶17 mRNA和蛋白在食管鳞癌组织中的表达及其临床意义
- Author:
Hong-bin LIU
1
;
Qi-chang YANG
;
Yi SHEN
;
Yan ZHU
;
Xiao-juan ZHANG
;
Hao CHEN
Author Information
- Publication Type:Journal Article
- MeSH: ADAM Proteins; genetics; metabolism; ADAM17 Protein; Aged; Biomarkers, Tumor; metabolism; Carcinoma, Squamous Cell; metabolism; pathology; surgery; Esophageal Neoplasms; metabolism; pathology; surgery; Female; Follow-Up Studies; Humans; Kaplan-Meier Estimate; Lymphatic Metastasis; Male; Middle Aged; Neoplasm Staging; Proportional Hazards Models; RNA, Messenger; metabolism; Receptor, Epidermal Growth Factor; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Survival Rate
- From: Chinese Journal of Oncology 2013;35(5):361-365
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of A disintegrin and metalloproteinase 17 (ADAM17) mRNA and ADAM17 protein in esophageal squamous cell carcinoma (ESCC), and to evaluate their correlation with clinicopathological features and prognosis.
METHODSThe expression of ADAM17 mRNA in 50 ESCC and 50 normal esophageal tissues was detected by RT-PCR. The expression of ADAM17 protein in 80 ESCC and 80 normal esophageal tissues was detected with immunohistochemioal staining (SP). Log rank test and Cox proportional hazards analysis were used to analyze the prognosis of ESCC.
RESULTSThe expression of ADAM17 mRNA in 50 ESCC and 50 normal esophageal tissues was 0.937 ± 0.241 and 0.225 ± 0.077, respectively. The positive expression rates of ADAM17 protein in 80 ESCC and 80 normal esophageal tissues was 66.2% and 6.2%, respectively. The expressions of ADAM17 mRNA and ADAM17 protein in the ESCC were significantly higher than those in normal esophageal tissues (P < 0.01). The expressions of ADAM17 mRNA and protein were positively correlated with lymph node metastasis and TNM staging (P < 0.05). There were no correlations between the expressions of ADAM17 mRNA and protein and sex, age and histological grade (P > 0.05) . The expression of ADAM17 protein was positively correlated with EGFR protein (P < 0.01). The lymph node metastasis, TNM staging and the expression of ADAM17 and EGFR protein were independent prognostic factors.
CONCLUSIONSADAM17 mRNA and protein are highly expressed in ESCC than in normal esophageal tissues and may play an important role in the development, invasion and metastasis of ESCC. They may be used as prognostic factors of ESCC.
