Bone marrow-derived dendritic cells rather than spleen-derived dendritic cells can generate regulatory T cells.
10.7534/j.issn.1009-2137.2013.04.039
- Author:
Hai-Tao ZHOU
1
;
Xian-Zhang HUANG
;
Xin-Zhong WU
;
Wei-Ye CHEN
;
Xiao-Bin WU
Author Information
1. Medical Blood Laboratory, Department of Laboratory Medicine, Guangdong Provincial Hospital of Traditional Chinese Medical, Guangzhou 510120, Guangdong Province, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Cells;
cytology;
Cell Differentiation;
Cells, Cultured;
Coculture Techniques;
Dendritic Cells;
cytology;
Female;
Forkhead Transcription Factors;
metabolism;
Mice;
Mice, Inbred BALB C;
Mice, Inbred C57BL;
Spleen;
cytology;
T-Lymphocytes, Regulatory;
cytology
- From:
Journal of Experimental Hematology
2013;21(4):1015-1020
- CountryChina
- Language:Chinese
-
Abstract:
There are many evidences that dendritic cells (DC) can establish and maintain immunological tolerance through inducing the differentiation of regulatory T cells Treg. This study was purposed to explore the possibility to gene-rate Treg from bone marrow-derived DC (BM-DC) or spleen-derived DC (spDC) generated CD4(+) CD25(+) FOXP3(+) Treg by induction. Bone marrow immature DC (imDC) induced from bone marrow precursor cells of C57BL/6 mice by GM-CSF and IL-4; after culture for 6 day, imDC were stimulated by LPS for additional 16 hours and the mature DC (mDC) have been got; the spDC were collected from spleen of C57BL/6 mice by MACS. Co-culturing fresh BALB/c mouse CD4(+) T cells with these three sorts of DC above mentioned respectively was performed to generate CD4(+) CD25(+) FOXP3(+) Treg. The expression of FOXP3 in CD4(+) T cells was detected by flow cytometry, and the capacity of different DC generated CD4(+) CD25(+) FOXP3(+) Treg was evaluated. The results showed that stimulated by C57BL/6 immature or mature DC, the positive rate of FOXP3 in BALB/c CD4(+) T cells increased from (8.57 ± 1.14)% to (15.80 ± 1.35)%, (17.93 ± 1.45)% respectively (P < 0.01); while stimulated by spDC, the positive rate of FOXP3 in BALB/c CD4(+) T cells decreased from (8.57 ± 1.14)% to (3.95 ± 0.79)% (P < 0.05). It is concluded that the BM-DC but not spDC can generate Treg from CD4(+) T cells, BM-DC may mediate immune tolerance rather than the immune response.
