Cloning of full-length cDNA sequence of a novel candidate gene related to mouse cleft palate.
- Author:
Xin LI
1
;
Yan JIN
;
Wei YAN
;
Yuan LIU
;
Xin-wen WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adaptor Proteins, Signal Transducing; genetics; Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; Cleft Palate; genetics; pathology; Cloning, Molecular; DNA, Complementary; chemistry; genetics; Female; Genetic Predisposition to Disease; Mice; Mice, Inbred C57BL; Molecular Sequence Data; Nucleic Acid Hybridization; methods; Polymerase Chain Reaction; Pregnancy; Sequence Analysis, DNA
- From: Chinese Journal of Medical Genetics 2005;22(5):481-484
- CountryChina
- Language:English
-
Abstract:
OBJECTIVECloning and screening a novel candidate gene related to developing mouse cleft palate.
METHODSThe differentially expressed genes were cloned by modified PCR-based subtractive hybridization. After identification using reverse dot blotting, positive clones were sequenced and analyzed for homology in GenBank databases.
RESULTSFour novel express sequence tags were obtained, one of which spanning 809 bp was the full-length of the novel gene cDNA identified by Northern hybridization.
CONCLUSIONA novel candidate gene related to mouse cleft palate was cloned.
