Evaluation for extraction process of Shenqi Tongmai Yizhi particles based on antioxidant capacity in vitro and its spectrum-effect relation.
- Author:
Xiao-Li ZHANG
1
;
Yu-Ling LIU
1
;
Li-Jiao FAN
1
;
Yue-Liang WANG
1
;
Kai CHEN
1
;
Hui LI
1
Author Information
- Publication Type:Journal Article
- Keywords: DPPH; antioxidant; fingerprint; grey relational analysis; spectrum efficiency relations
- From: China Journal of Chinese Materia Medica 2016;41(9):1635-1639
- CountryChina
- Language:Chinese
- Abstract: Based on DPPH method, the antioxidant activities of Shenqi Tongmai Yizhi particles with different extraction processes were compared. The contribution to the anti-oxidant capacity in vitro was explored by means of grey relational analysis on different chemical compositions in the fingerprint. The results showed that the IC₅₀ concentration values of water extract, water extract from alcohol precipitation, alcohol extract, and alcohol and water extract were 0.801 4, 0.859 1, 0.796 1, 0.918 0 g•L⁻¹; and the alcohol extract is the best method to extract antioxidative components, with the highest antioxidant activity and lowest IC₅₀. When the mass concentration of the herbs reached a certain degree, its free radical clearance rate was similar to that of vitamin C control group. The order of different chemical contributions of constituents to the antioxidant activity in the fingerprint was 4>3>33>53>9>10>11>34>15>59>8>61>52>20>42>18>29. The preliminary exploration for the spectrum efficiency relations provides reference for studying traditional Chinese medicine compound processing method and the pharmacodyamic material basis.
