Advances in the MDCK-MDR1 cell model and its applications to screen drug permeability.
- Author:
Yao LIU
1
;
Su ZENG
Author Information
1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
- Publication Type:Journal Article
- MeSH:
ATP-Binding Cassette, Sub-Family B, Member 1;
metabolism;
Animals;
Biological Transport;
Blood-Brain Barrier;
metabolism;
Caco-2 Cells;
Cell Line;
Cell Membrane Permeability;
Dogs;
Drug Evaluation, Preclinical;
Drug Resistance, Multiple;
Genes, MDR;
Humans;
Intestinal Absorption;
Intestinal Mucosa;
metabolism;
Pharmaceutical Preparations;
metabolism
- From:
Acta Pharmaceutica Sinica
2008;43(6):559-564
- CountryChina
- Language:Chinese
-
Abstract:
This article introduces the characteristics and establishment of MDCK-MDR1 cell line, and its applications as an in vitro model for the assessment of the membrane permeability properties of drugs. MDCK-MDR1 cell model is widely used in drug screenings, and the study of mechanisms of drug interaction and absorption or transport. The review summarizes the progress and applications of MDCK-MDR1 cell model. Because there is high polarized P-glycoprotein (P-gp) expression in MDCK-MDR1 cells, it could be used as a drug transport model to screen P-gp substrates or inhibitors, as well as models of intestinal mucosa, blood brain barrier and kidney. Although there are some disadvantages in MDCK-MDR1 cell model, it is an effective tool for implementing permeability of drug in an optimal fashion.
