Effect of Tongxie Yaofang on endogenous metabolites in serum of IBS model rats.
10.19540/j.cnki.cjcmm.20170121.016
- Author:
Kai LI
1
;
Hai-Xue KUANG
1
;
Yue YIN
1
;
Jie-Yu ZHANG
2
;
Zhi WANG
3
;
Qiu-Yue ZHANG
1
;
Jian-Wei WANG
1
Author Information
1. Heilongjiang University of Chinese Medicine, Harbin 150040, China.
2. First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040, China.
3. Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150001, China.
- Publication Type:Journal Article
- Keywords:
IBS;
Tongxie Yaofang;
endogenous metabolites;
liver stagnation and spleen deficiency;
metabolomics
- From:
China Journal of Chinese Materia Medica
2017;42(5):970-981
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate the effect of Tongxie Yaofang on cardiac endogenous metabolism in irritable bowel syndrome(IBS) rats by using metabolomics method, find its potential biomarkers, analyze the metabolic pathways, and explore the pharmacological effects, mechanisms of action and syndrome essence of syndrome model. Forty Wistar rats were used to establish IBS models, and then randomly divided into four groups： model control group and Tongxie Yaofang treatment groups (high, medium, low dose). Another 10 rats were used as normal group. The rats in Tongxie Yaofang-treated(low, medium and high dose) groups were orally administrated with Tongxie Yaofang extracts once a day for 2 weeks, respondingly with the doses of 0.203,0.406,0.812 g•mL⁻¹. The rats in normal group and model control group were given with equal volume of saline once a day for 2 weeks. On the 0 and 15th days, serum was collected and each sample extract was analyzed by UPLC-Q-TOF-MS. Eight potential biomarkers were identified and 8 major metabolic pathways were found to be related with IBS diseases neurotransmitter metabolism, inflammatory immunity, brain function and energy metabolism, etc. Tongxie Yaofang had certain pharmacological effects on IBS, and its mechanism may be related to serotonergic synapse, tryptophan metabolism, cysteine and methionine metabolism, glycerophospholipid metabolism, nicotinate and nicotinamide metabolism and so on, which might be the biological basis of IBS liver-spleen deficiency syndrome.
