Fast identification of constituents of Lagotis brevituba by using UPLC-Q-TOF-MS/MS method.
10.19540/j.cnki.cjcmm.20170307.012
- Author:
Jing XIE
1
;
Li ZHANG
2
;
Jin-Xiang ZENG
1
;
Min LI
1
;
Juan WANG
1
;
Xiong-Xiong XIE
1
;
Guo-Yue ZHONG
1
;
Guang-Ming LUO
1
;
Jin-Bin YUAN
1
;
Jian LIANG
1
Author Information
1. Research Center of Traditional Chinese Medicine Resources and Minority Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
2. Department of Budget and Finance, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
- Publication Type:Journal Article
- Keywords:
Lagotis brevituba;
UPLC-Q-TOF-MS/MS;
chemical constituents;
fast identification
- From:
China Journal of Chinese Materia Medica
2017;42(11):2123-2130
- CountryChina
- Language:Chinese
-
Abstract:
The chemical constituents of Lagotis brevituba were rapidly determined and analyzed by using ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) method, providing material basis for the clinical application of L. brevituba. The separation was performed on UPLC YMC-Triart C₁₈ (2.1 mm×100 mm, 1.9 μm) column, with acetonitrile-water containing 0.2% formic acid as mobile phase for gradient elution. The flow rate was 0.4 mL•min-1 gradient elution and column temperature was 40 ℃, the injection volume was 2 μL. ESI ion source was used to ensure the data collected in a negative ion mode. The chemical components of L. brevituba were identified through retention time, exact relative molecular mass, cleavage fragments of MS/MS and reported data. The results showed that a total of 22 compounds were identified, including 11 flavones, 6 phenylethanoid glycosides, 1 iridoid glucosides, and 4 organic acid. The UPLC-Q-TOF-MS/MS method could fast identify the chemical components of L. brevituba, providing valuable information about L. brevituba for its clinical application.
