Experimental study of tissue-engineered biological small intestinal submucosa,membrane employed in dental implant traction
10.3760/cma.j.issn.1002-0098.2009.07.013
- VernacularTitle:组织工程化小肠黏膜下层生物膜用于牙种植牵引的实验研究
- Author:
Hua LI
1
;
Zheng-Xue HAN
;
Li-Qua XU
;
Chen-Ping ZHANG
Author Information
1. 首都医科大学口腔医学院
- Keywords:
Tissue engineering;
Biolilms;
Dental implantation;
Traction
- From:
Chinese Journal of Stomatology
2009;44(7):433-437
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the tissue-engineered biological small intestinal submucosa(SIS)membrane and evaluate the feasibility of its use as a surrogate of periosteum and its possible role in dental implant distraction(DID).Methods The tissue-engineered biological SIS inembrane was,constructed through the co-culturing of boat bone marrow stromal cells(BMSC)and small intestinal submucosa.The celluar compatibility was evaluated with the phase contrast microscope,SEM,alkaline phosphatase(ALP)activity and histology,and its effect of ostcogenesis promotion was detected by Micro-CTand histology after implanted in the export side of DID operation.Results The phase contrast microscope,SEM,ALP activity and histology confirmed that the BMSC could adhere to SIS and proliferate on it normally,the cellular activity and function were not affected by SIS.Three months after the tissue-engineered biological SIS membrane was implanted into the exposed side,sonle discontinuous new bone in the"biological SIS membrane"group was detected by the Micro-CT under a higher window level.The histology revealed that there was a quantity of new bone in the distracted region and the majority was woven bone.The quantity and quality ofthe new bone in the"biological SIS membrane"implanted group were similar to the unexposed side,but the bone nonunion was detected in the un-implanted group and the interspace was fixed by fibrous connective tissue.Conclusions The tissueengineered biological SIS membrane can provid both daughter cells,guide bone regeneration in DID and promote the osteogenesis.But the outcome of the biological SIS membrane,especially the function of the osteoblast culmred in the SIS in new bone formation,still needs further study.
