Expression of triggering receptors expressed on myeloid-1 in innate response to Porphyromonas gingivalis in macrophages
10.3760/cma.j.issn.1002-0098.2013.12.007
- VernacularTitle:髓系细胞触发受体1在巨噬细胞应答牙龈卟啉单胞菌免疫反应中的表达
- Author:
Lang LEI
1
;
Hou-Xuan LI
;
Sheng-Bo PAN
;
Fu-Hua YAN
Author Information
1. 福建医科大学附属口腔医院·福建省高校口腔医学重点实验室
- Keywords:
Porphyromonas gingivalis;
Macrophages;
Triggering receptors expressed on myeloid-1
- From:
Chinese Journal of Stomatology
2013;48(12):730-733
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of triggering receptors expressed on myeloid-1 (TREM-1) in innate response to Porphyromonas gingivalis(Pg) in mice macrophages and its potential role in periodontitis development.Methods Peritoneal macrophages from mice were harvested,separated and cultured,then challenged with viable Pg.Transcription and protein expression in macrophages were assessed with real time PCR and flow cytometry respectively.LP-17 peptide (10,100 and 1000 μg/L) was utilized to block TREM-1,and tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were detected by enzyme linked absorbent analysis.Results At 2 h after Pg challenge,transcription of TREM-1 was significantly upregulated after Pg challenge [(7.99 ± 1.11) fold vs blank].At 24 h after bacteria infection,increased TREM-1 expression was demonstrated by flow cytometry,with mean fluorescent intensity increasing from (7.05 ± 1.85) in blank group to (13.17 ± 2.33) in experimental group.Proinflammatory cytokine(TNF-α and IL-6) production was significantly decreased after blocking TREM-1 by LP-17 peptide (100 and 1000 μg/L).Conclusions TREM-1 enhanced innate immune response to Pg in macrophages,which may facilitate periodontitis development.
