Influence of zoledronate on osteoclast differentiation and gene expression of calmodulin and calmodulin-dependent protein kinase Ⅱ
10.3760/cma.j.issn.1002-0098.2013.11.012
- VernacularTitle:唑来膦酸对破骨细胞分化中钙调蛋白和钙调蛋白依赖性激酶Ⅱ基因表达的影响
- Author:
Peng LI
1
;
Jue-Shan LIN
;
Peng ZHANG
;
Wei DONG
;
Jin-Yuan LI
;
Meng-Chun QI
Author Information
1. 河北联合大学口腔医学院口腔颌面外科
- Keywords:
Biphosphonates;
Osteoclasts;
calmodulin;
calcium/calmodulin-dependent protein kinase
- From:
Chinese Journal of Stomatology
2013;48(11):694-698
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of zoledronate acid on osteoclast differentiation and gene expression of calmodulin (CAM) and calmodulin-dependent protein kinase (CAMK) Ⅱ.Methods Receptor activatior of nuclear factor κB ligand (RANKL) was used to induce differentiation of RAW264.7cells into osteoclasts in vitro.The cells were divided into two groups,group A and group B.Both groups were treated with RANKL for 5 days,whereas group B was also treated with zoledronate for the last 2 days.Osteoclastogenesis and gene expression of CAM and CAMK Ⅱ were examined.Results In group B,the number of new-generated osteoclasts (≥ 3 nuclei),number and size of dentin resorption lacunaes were (23 ±3),(19 ±2) and (4951 ±223) μm2 respevtively,which were significantly lower than those [(44 ±3),(46±1) and (13 331 ±248) μ m2]in group A (P＜0.01).mRNA and protein level of CAM and CAMK Ⅱ were also significantly down-regulated in group B when compared with group A (P ＜ 0.01) and the decrease was 26.7％ and 37.2％ respectively for CAM,57.0％ and 76.1％ respectively for CAMK Ⅱ.Conclusions Zoledronate acid could significantly inhibit formation and resorption function of osteoclasts.CAM and CAMK Ⅱ may be involved in the inhibition process.
