Sequence Analysis of A Novel HLA-B*13:68 Allele.
- Author:
Bin HAN
1
;
Li HAN
1
;
Zhi-Hui FENG
1
;
Xiao-Yun CHI
1
;
Shu-Xian JIAO
2
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Asian Continental Ancestry Group; Base Sequence; HLA-B Antigens; Histocompatibility Testing; Humans; Polymerase Chain Reaction; Sequence Analysis, DNA
- From: Journal of Experimental Hematology 2015;23(4):1144-1146
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe purpose of this research was to identify a novel HLA-B allele in Chinese population.
METHODSThe HLA typing of bone marrow donors was performed by PCR-SBT. The ambiguous novel HLA allele was confirmed with GSSP method.
RESULTSThe sequence of a sample was different from all alleles in the HLA-B databases. The sequence analysis showed that it differed from the closet matching allele HLA-B*13:01:01 at one nucleotide substitution, 137 T > C in Exon2, which resulted in an amino acid change from Phenylalanine (Phe) to Serine (Ser) at codon 22.
CONCLUSIONA novel allele was identified and named as HLA-B*13:68 by the WHO Nomenclature Committee.
