Study on UHPLC fingerprint and determination of eight phenolic components of Tetrastigma hemsleyanum leaves.
- Author:
Shi-Ming FAN
1
;
Hui-Long XU
1
;
Xin-Yue XIE
1
;
Bi-Ya CAI
1
;
Fu-Xian ZOU
1
;
Wen XU
1
;
Zhi-Sen XIE
2
;
Xin-Ping HUANG
2
Author Information
- Publication Type:Journal Article
- Keywords: Tetrastigma hemsleyanum leaves; UHPLC; fingerprint analysis; quality control
- From: China Journal of Chinese Materia Medica 2016;41(21):3975-3981
- CountryChina
- Language:Chinese
- Abstract: A novel method combining ultra-high performance liquid chromatography (UHPLC) fingerprint and simultaneous quantitative analysis of eight phenolic components was developed and validated for quality evaluation of Tetrastigma hemsleyanum leaves. For fingerprint analysis, 15 peaks were selected as the common peaks to evaluate the similarities among 41 batches of T. hemsleyanum leaves collected from different regions. Additionally, simultaneous quantification of eight markers, including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isoorientin, orientin, vitexin-2-O-rhamnoside,vitexin and isovitexin, was performed and the obtained data demonstrated that our method has achieved desired linearity, precision and accuracy. Clustering statistical analysis was further application in T. hemsleyanum leaves from different regions. The results indicated that new approach conbine ultra-high performance liquid chromatography (UHPLC) fingerprint and simultaneous quantitative analysis of eight phenolic components was applicable in quality control of T. hemsleyanum leaves.
