Simultaneous determination of 8 main compounds in Eclipta Herba by UPLC.
- Author:
Xue-Feng HOU
1
;
Shuai SUN
1
;
Gang WANG
1
;
Hui-Hui QIU
2
;
Shu-Min DING
3
;
Ying LIU
1
;
Dan LIU
2
;
Liang FENG
2
;
Xiao-Bin JIA
2
Author Information
- Publication Type:Journal Article
- Keywords: Eclipta Herba; UPLC; content determination; hyperuricemia; multi-component quantification
- From: China Journal of Chinese Materia Medica 2016;41(21):3982-3987
- CountryChina
- Language:Chinese
- Abstract: To establish an UPLC method for the simultaneous determination of 8 compounds in Eclipta Herba, such as isoquercitrin, luteoloside, demethylwedelolactone, isochlorogenic acid A, isochlorogenic acid C, luteolin, wedelolactone and apigenin. The experiment was performed with a Waters Acquity UPLC BEH C₁₈ (2.1 mm×100 mm, 1.7 μm) column by gradient elution of 0.1% formic acid in water and acetonitrile: 0-4 min,10%-13% A; 4-10 min, 13%-16% A; 10-13 min, 16%-25% A; 13-17 min, 25%-28% A; 17-20 min,28%-40% A;20-25 min,40%-95% A. The flow rate was 0.3 mL•min⁻¹.. The condition of was the colum temperature was maintained at 35 ℃ and the detected wavelength was set at 350 mm. 8 components were separated clearly by this method. Also a good linearity was obtained between the chosen concentration(r≥0.999 0). The measured data showed that the recovery rate range from 96.60%-103.4% (n=6) and their RSD values were 0.86%-2.4%. The method has high recovery rate, good reproducibility and stability. It provides a scientific basis for the identification and quality evaluation of Eclipta Herba.
