Transcriptome characterization of Ephedra sinica with 454 ESTs.

Hai-Xia YAN; Hong-Mei LUO; Ying LI; Yong-Zhen SUN; Chao SUN; Zhong-Zhi QIAN; Shi-Lin CHEN

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Transcriptome characterization of Ephedra sinica with 454 ESTs.

Author: Hai-Xia YAN ¹ ; Hong-Mei LUO ¹ ; Ying LI ¹ ; Yong-Zhen SUN ¹ ; Chao SUN ¹ ; Zhong-Zhi QIAN ² ; Shi-Lin CHEN ³
Author Information

1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
2. Chinese Pharmacopoeia Commission, Beijing 100061, China.
3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Publication Type:Journal Article
Keywords: 454 GS FLX; Ephedra sinica; expressed sequence tags; transcriptome
From: China Journal of Chinese Materia Medica 2016;41(22):4158-4164
CountryChina
Language:Chinese
Abstract: Using the latest 454 GS FLX platform and Titanium regent, a substantial expressed sequence tag (ESTs) dataset of Ephedra sinica was produced, and the profile of gene expression and function gene of which were investigated. A total of 48 389 reads with an average length of 373 bp were generated. These 454 reads were assembled into 18 801 unigenes, which were all 454 sequencing identified. A total number of 10 531 unigenes(56.0%) were annotated using BLAST searches (E-value≤1×10⁻⁵) against the Nr, Nt, TAIR, SwissProt and KEGG databases. With respect to genes related to ephedrine biosynthesis, 19 unigenes(encoding 9 enzymes) were found. A total of 97 putative genes encoding cytochrome P450s were also discovered. Data presented in this study will provide an important resource for the scientific community that is interested in the functional genomics and secondary metabolism of E. sinica.