In vitro screening and identification of plant growth-promoting rhizobacteria against pathogen causing Astragalus root rot.

Fen GAO; Rui HAO; Xue-Mei QIN; Zhen-Hong LEI; Yu-Long WANG; Meng-Liang WANG

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In vitro screening and identification of plant growth-promoting rhizobacteria against pathogen causing Astragalus root rot.

Author: Fen GAO ¹ ; Rui HAO ² ; Xue-Mei QIN ³ ; Zhen-Hong LEI ⁴ ; Yu-Long WANG ⁴ ; Meng-Liang WANG ¹
Author Information

1. Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China.
2. Institute of Biotechnology, Shanxi University, Taiyuan 030006, China.
3. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China.
4. Shanxi Zhendong Geo-herbals Development Co., Ltd., Changzhi 047100, China.
Publication Type:Journal Article
Keywords: Astragalus root rot; identification; plant growth promoting rhizobacteria(PGPR); screening
From: China Journal of Chinese Materia Medica 2016;41(22):4188-4193
CountryChina
Language:Chinese
Abstract: The antagonistic effect of Bacillus spp. against Fusarium solani was evaluated by living body dual culture and Oxford cup method. The plant growth promoting properties of those strains that had obvious and stable antifungal activity were then tested. The results showed that the living body and bacteria-free fermentation filtrate of strain G10 both had obvious and stable antifungal effect to F. solani. Besides, the strain possessed such growth promoting properties as phosphate solubilization, nitrogen fixation, and production of IAA, amylase and HCN. Strain G10 was classified and identified as B. subtilis by a combination of morphological, physiological and biochemical tests, 16 SrDNA gene sequence analysis and the BBL CrystalTM bacteria identification. In conclusion, B. subtilis G10 has the basic characteristics of multifunctional strains and could be one of the microbiological resources for developing special bio-control agent against Astragalus root rot.