Inductive Effect of 5-Azacitidine on Apoptosis of Multiple Myeloma Cell lines and Its Mechanism.
- Author:
Wen-Ming WANG
1
;
Jing WANG
1
;
Ming-Xia ZHU
1
;
Yan-Fang WANG
1
;
Yuan-Yuan LIU
1
;
Hong-Mei JING
2
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Azacitidine; pharmacology; Caspase 3; metabolism; Cell Cycle; Cell Cycle Checkpoints; Cell Line, Tumor; drug effects; Cell Proliferation; Flow Cytometry; Humans; MAP Kinase Signaling System; drug effects; Multiple Myeloma; pathology; Proto-Oncogene Proteins c-bcl-2; metabolism; bcl-2-Associated X Protein; metabolism
- From: Journal of Experimental Hematology 2016;24(1):110-116
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the response of multiple myeloma (MM) cells to 5-azacitidine and its possible mechanisms.
METHODSTwo multiple myeloma-derived cell lines U266 and H929 were used in this study. The cell proliferation and apoptosis were assessed by CCK-8, flow cytometry and acridine orange staining. The cell cycle was assessed by flow cytometry. The expression of BCL-2, BAX was assessed by RT-PCR. The expressions of caspase-3 and p-ERK1/2 were assessed by Western blot.
RESULTSThe BCL-2/BAX ratio decreased, the activity of caspase-3 and p-ERK1/2 increased, the cell cycle was arrested in G2/M phase after treatment with 5-azacitidine. The 5-azacitidine inhibited proliferation in a dose-dependent manner.
CONCLUSION5-azacitidine exerts apoptosis-inducing and grow-inhibiting effects on MM cell lines, its mechanism may be related with the decrease of BCL-2/BAX ratio, caspase-3 activation and the arrest of cell cycle.
