Inhibitory effect of lentiviral vectors expressing small interfering RNA targeting survivin gene on Hep-2 cell growth in vitro and in nude mice.
- Author:
Xiao-bao YAO
1
;
Xiao-xia WANG
;
Shao-qiang ZHANG
;
Li-ying YAN
;
Hong-liang ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; genetics; Cell Line, Tumor; Gene Knockdown Techniques; Genetic Vectors; genetics; Humans; Inhibitor of Apoptosis Proteins; biosynthesis; genetics; Laryngeal Neoplasms; genetics; pathology; Lentivirus; genetics; metabolism; Mice; Mice, Inbred BALB C; Mice, Nude; RNA, Messenger; biosynthesis; genetics; RNA, Small Interfering; genetics; Repressor Proteins; biosynthesis; genetics; Transfection
- From: Journal of Southern Medical University 2010;30(1):170-172
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of the lentiviral vectors expressing small interfering RNA (siRNA) for survivin gene knockdown in inhibiting Hep-2 cell growth in vitro and its tumorigenicity in nude mice.
METHODSThe tumorigenicity of Hep-2 cells transfected with the siRNA mediated by the lentiviral vectors was tested in nude mice. The expression of survivin gene of the transfected cells at the mRNA and protein levels were detected by RT-PCR and Western blotting, respectively, and the cell cycle changes were analyzed by flow cytometry.
RESULTSTransfection of the siRNA targeting survivin significantly decreased the expression of survivin mRNA and protein in Hep-2 cells in vitro by 60%-85% and 70%, respectively, resulting also in increased cell apoptosis as shown by flow cytometry (P<0.01). The transfection significantly lowered the tumorigenicity of the cells in nude mice.
CONCLUSIONThe lentiviral vectors expressing survivin siRNA can significantly inhibit survivin gene expression in Hep-2 cells and induce the cell apoptosis in vitro, and suppress the tumorigenicity of the cells in nude mice.
