Mechanisms for inhibition effects of polypeptide extract from scorpion venom (PESV) on proliferation of A549 cell lines in vitro.
- Author:
Xiaohui WANG
1
;
Zhaopeng WANG
;
Yueying ZHANG
;
Qing JIA
;
Zhaoxia WANG
;
Jie ZHANG
;
Weidong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; isolation & purification; pharmacology; Cell Cycle; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Gene Expression Regulation, Neoplastic; drug effects; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; metabolism; PTEN Phosphohydrolase; metabolism; Peptides; isolation & purification; pharmacology; Scorpion Venoms; chemistry; Vascular Endothelial Growth Factor A; metabolism
- From: China Journal of Chinese Materia Medica 2012;37(11):1620-1623
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mechanisms for inhibition effects of PESV on proliferation of non-small cell lung cancer cell line A549.
METHODMTT was used to observe cell growth and proliferation of A549 at different concentrations of PESV. Flow cytometry (FCM) was applied to analyze cell cycle distribution. Immunocytochemistry and western blot assay was recruited to detect the expression of VEGF, HIF-1alpha, PTEN after the intervention of PESV.
RESULTA549 cells may be arrested mainly in G0/G1 phase and cell proliferation was significantly inhibited (P < 0.01) after PESV intervention in a certain range of concentration. PESV can significantly reduce the expression of HIF-1alpha,VEGF and increase the expression of PTEN.
CONCLUSIONPESV can block cell cycle and inhibit angiogenesis directly to inhibit cell proliferation of non-small cell lung cancer cell line A549 mainly through reducing the expression of HIF-1alpha, VEGF and increasing the expression of PTEN.
