Optimization of conditions for human erythropoiesis in human-mouse xenotransplant model.
10.7534/j.issn.1009-2137.2013.06.045
- Author:
Wei-Wei ZHENG
1
;
Fei-Fei XU
2
;
Rong-Hua YIN
1
;
Yi-Qun ZHAN
1
;
Xiao-Ming YANG
1
;
Zhang-Yan LI
3
,
4
Author Information
1. Department of Biochemistry and Molecular Biology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
2. Department of Biochemistry and Molecular Biology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China
3. Department of Biochemistry and Molecular Biology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China. E-mail: fmmli@
4. com.
- Publication Type:Journal Article
- MeSH:
Animals;
Antigens, CD34;
Bone Marrow Cells;
cytology;
Cells, Cultured;
Erythrocytes;
cytology;
Erythropoiesis;
Female;
Hematopoietic Stem Cell Transplantation;
methods;
Humans;
Mice;
Mice, Inbred NOD;
Mice, SCID;
Transplantation, Heterologous
- From:
Journal of Experimental Hematology
2013;21(6):1606-1611
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the conditions for improving human-mouse xenograft and the erythroid differentiation of human hematopoietic stem cells (HSC) in the xenotransplant model. The engraftments of different mouse strains (NOD/SCID or NOD/SCID/IL2rγ(null)), schemes of irradiation (single-time or 2-times radiation; Co(60)γ-ray or X-ray) and strategies of CB CD34(+) cells ex vivo culture time and lentivirus infection were compared. The results showed that at 4 weeks after transplantation, the ratio of hCD45 positive cells in bone marrow of NOD/SCID/IL2rγ(null) mice increased to (51.4 ± 13.9)%, and erythroid precursor could be detected. All of the mice receiving X-ray irradiation for 2 times (a dose of 1 Gy, then the second of 1.5 Gy, with an interval of 15 min) survived. Fresh isolated CB CD34(+) cells were cultured and infected with lentivirus for 72 h and then transplanted into receptor mouse. After 4 weeks, higher engraftment [hCD45 (51.4 ± 13.9)%] and better erythroid development [hCD71(+) GPA(+) (5.98 ± 3.46)%] were observed. It is concluded that NOD/SCID/IL2rγ(null) mice receiving X-ray irradiation for 2 times and were injected with fresh isolated CB CD34(+) cells cultured and infected with lentivirus ex vivo within 72 h show a better xenograft and erythroid development.
