OBJECTIVETo study the effect of retinoic acid (RA) on cell proliferation and apoptosis of palatal shelves by 5-bromo-2-deoxyuridine (BrdU) and terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL).

METHODSC57BL/6J mice were used to establish the RA induced cleft palate animal model, in which the pregnant mice were given a single dose of RA at 100 mg/kg body weight on gestation day 10 (GD10) and GD12, respectively. Specimens were prepared for immunohistochemical staining by using BrdU and TUNEL monoclonal antibodies.

RESULTSThe percentages of BrdU positive cells of embryonic palatal mesenchyme (EPM) and medial edge epithelium (MEE) in GD10 RA group were both obviously lower than that of control group. There was no difference between GD12 RA group and control group. Abnormally intense staining of TUNEL was detected in the palatal mesenchymal cells of GD10 day RA group but not in control group and GD12 day RA group.

CONCLUSIONAfter exposure of embryonic mice to RA on GD10, the proliferation and apoptosis of palatal mesenchymal cells are increased, this causes the smaller size of shelves and failure of fusion. The MEE cells keep a bilayer midline epithelial seam after exposure on GD12 with normal apoptosis, this indicates that cell apoptosis in MEE cells be not the only process required for palatal fusion.