Effects of GBE50 on physiological characteristics and intracellular free calcium of myocardium.
- Author:
Xingyu WANG
1
;
Zhixiong ZHANG
;
Aihua LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium; metabolism; Cells, Cultured; Ginkgo biloba; chemistry; Guinea Pigs; Heart; drug effects; physiology; In Vitro Techniques; Male; Myocardial Contraction; drug effects; Myocardium; metabolism; Myocytes, Cardiac; drug effects; metabolism; Plant Extracts; pharmacology
- From: China Journal of Chinese Materia Medica 2010;35(14):1866-1870
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate effects of GBE50 on the physiological characteristics and intracellular free calcium of myocardium.
METHODThe preparations of isolated atria of guinea pigs were used for recording the myocardial physiological characteristics and the technique of Fluo-3/AM was used for measuring the concentration of free calcium in myocytes.
RESULTGBE50 (10, 20, 40, 80, 160 mg x L(-1)) prominently inhibited the contractive force and decreased spontaneous beat of right atrium in concentration-dependent manner. GBE50 (10, 20, 40, 80, 160 mg x L(-1)) inhibited the contractive force of left atrium, and markedly decreased the post rest potentiation of myocardial contractility in left atrium in concentration-dependent manner. GBE50 (10, 20, 40, 80, 160 mg x L(-1)) prolonged the function refractory period of isolated guinea pig left atrium. 50 mg x L(-1) GBE50 decreased [Ca2+]i of guinea pig ventricular myocytes in normal Tyrode's solution by (46.53 +/- 8.44)% in 12 minutes.
CONCLUSIONGBE50 induces inhibitory effects of the spontaneous beat, contractility, post-rest potentiation and prolonged the function refractory period. GBE50 may decrease [Ca2+]i of guinea pig ventricular myocytes.
