Effect of KH2 PO4 on the odonto-and osteogenic differentiation potential of human stem cells from apical papillae
10.3760/cma.j.issn.1002-0098.2013.01.008
- VernacularTitle:磷酸二氢钾对根尖牙乳头干细胞成牙及成骨向分化能力的影响
- Author:
Yan-Ping WANG
1
;
Jin-Tao WU
;
Zi-Lu WANG
;
Yang-Yu ZHENG
;
Guang-Dong ZHANG
;
Jin-Hua YU
Author Information
1. 210029,南京医科大学口腔医学研究所 南京医科大学附属口腔医院牙体牙髓病科
- Keywords:
Stem cells research;
Potassium dihydrogen phosphate;
Cell differentiation
- From:
Chinese Journal of Stomatology
2013;48(1):27-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effects of KH2PO4 on the odonto-and osteogenic differentiation potential of human stem cells from apical papillae (SCAP) in vitro.Methods SCAP were isolated and cultured respectively in alpha minimum essential medium (α-MEM) or α-MEM containing 1.8 mmol/L KH2PO4.Alkaline phosphotase(ALP) activity,alizarin red staining,real-time reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to examine the odonto and osteogenic potential of SCAP in the two media.Results SCAP cultured in α-MEM containing 1.8 mmol/L KH2PO4 exhibited a higher ALP activity [(0.370 ± 0.013) Sigma unit · min-1 · mg-1] at day 3 than control group [(0.285±0.008) Sigma unit · min-1 · mg-1] and KH2PO4-treated SCAP formed more calcified nodules at day 5 [(0.539 ± 0.007) μg/g] and day 7 [(1.617 ± 0.042) μg/g] than those in normal medium[(0.138 ±0.037) μg/g,P ＜0.01].The expression of odonto-and osteogenic markers were significantly up-regulated after the stimulation of KH2P04 at day 3 and 7 respectively,as compared with control group.Conclusions 1.8 mmol/L KH2PO4 can promote the odonto and osteogenic differentiation potential of human SCAP.
