Effect of experimental occlusal hypofunction on the three-dimensional microarchitecture of mandibular bone in rats
10.3760/cma.j.issn.1002-0098.2013.01.014
- VernacularTitle:咬合功能减退对大鼠下颌骨影响的三维显微结构观察
- Author:
Guo-Feng ZHOU
1
;
Li-Xin XIANG
;
Hong LI
Author Information
1. 浙江医院口腔科
- Keywords:
X-ray microtomography;
Mandible;
Hypofunction
- From:
Chinese Journal of Stomatology
2013;48(1):53-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of experimental occlusal hypofunction on the architecture of rat' s mandibular bone.Methods Forty Sprague-Dawley (SD) male rats were randomly divided into experimental occlusal hypofunction group and control group,with 20 rats in each group.Experimental occlusal hypofunction group rats were fitted with a metal cap to the maxillary and mandibular incisor,to eliminate the occlusal forces on rat molars.In control group,a metal ring was fitted to the maxillary and mandibular incisor to maintain molar occlusal contact.Then the rats were killed under deep anaesthesia at 0 week and at the end of 2,4,6,and 8 weeks,and the mandibles were immediately removed for measurement of the three-dimensional architecture of cancellous bone in the alveolar process with micro-CT.Results At the end of week 4,the bone volume/total volume (BV/TV) in the hypofunction group decreased compared with the control group [control group:(0.65 ± 0.05) ％,experimental group:(0.60 ± 0.05) ％,P ＜ 0.05] and lasted until the end of the experiment [control group:(0.72 ± 0.06) ％,experimental group:(0.51 ± 0.07)％,P ＜ 0.01].The similar result was also observed in trabecula thickness (Tb.Th) in experimental animals[control group:(168 ± 15),(170 ± 25),(180 ± 18) μm,experimental group:(152 ± 20),(145 ± 19),(142 ± 15) μm,P ＜ 0.05].The trabecula spacing (Tb.Sp)in the hypofunction group increased from the end of week 4 to the end of week 8 compared with the matched control groups [control group:(264 ± 21),(284 ± 17),(282 ± 26) μm,experimental group:(306±30),(316 ± 18),(332 ± 18) μm,P ＜0.05].The trabecula number (Tb.N) of experimental animals decreased from the end of week 6 [control group:(3.59 ± 0.22) mm-1 experimental group:(3.03 ± 0.31) mm-1 P ＜ 0.05] to the end of week 8 [control group:(3.66 ± 0.24) mm-1,experimental group:(2.85 ± 0.18) mm-1,P＜0.01].Conclusions Experimental occlusal hypofunction can result in the change of mandibular alveolar process three-dimensional architecture.
