Effect of icariin on the expression of receptor activator nuclear factor-κB ligand and osteoprotegerin of human periodontal ligament cells
10.3760/cma.j.issn.1002-0098.2013.02.002
- VernacularTitle:淫羊藿苷对人牙周膜细胞表达核因子κB受体激活蛋白配体和护骨因子的影响
- Author:
Qian DING
1
;
Feng-Qiu ZHANG
Author Information
1. 100050,首都医科大学口腔医学院牙周科
- Keywords:
Epimedium;
Sonicated extracts of Porphyromonas gingivalis;
Human periodontal ligament cell;
Receptor activator nuclear factor-κB ligand
- From:
Chinese Journal of Stomatology
2013;48(2):68-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of icariin on the expression of receptor activator nuclear factor-κB ligand(RANKL) and osteoprotegerin(OPG) of human periodontal ligament cells(hPDLC)inhibited by sonicated extracts of Porphyromonas gingivalis(Pg).Methods hPDLC were extracted from 14 premolars extracted from 11 to 18-year-old patients for orthodontic purpose and cultured.The 7th passages of hPDLC were divided into six groups,each group was stimulated with 0,0.001,0.01,0.1,1 mg/L icariin and 50 mg/L sonicated extracts for 48 h.Reverse transcription polymerase chain reaction(RT-PCR) and Western blotting were used to detect the expression of RANKL and OPG.Results Pg extracts group expressed more RANKL than the control group(3.60 ±0.11 vs 1.08 ±0.19),OPG expression decreased(0.32 ±0.08 vs 1.01 ±0.08),and RANKL/OPG ratio significantly increased(11.20 ±0.13 vs 1.00 ±0.10).Compared with control group,RANKL and OPG expression changed in groups stimulated with 0.001,0.01,0.1,1 mg/L icariin.OPG expression was higher in 0.1 mg/L group than in the control (36.84 ± 0.05 vs 1.01 ± 0.08),RANKL/OPG ratio was significantly lower than the control group (0.04 ± 0.03 vs 1.00 ± 0.10)(P ＜0.01).Conclusions The concentration of 0.1 mg/L icariin can inhibit hPDLC expression of RANKL in sonicated extracts of Pg and promote OPG expression.
