IL-24 Expression at Maternal-fetal Interface and Its Roles in Trophoblast Invasion
10.1007/s11596-008-0418-9
- Author:
CHENG HONG
1
;
ZOU LI
Author Information
1. Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, China
- Keywords:
IL-24;
maternal-fetal interface;
TEV-1 cell line;
extravillous trophoblast;
invasion
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(4):456-459
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the expression of IL-24 at maternal-fetal interface and the roles in extravillous trophoblast (the TEV-1 cell line) invasion were examined. Immunohistochemistry was used to detect the expression of IL-24 in villi and decidual tissue. The proliferation of TEV-1 cells under the effect of IL-24 was measured by MTT assay. The invasiveness of TEV-1 cells under the effect of recombinant IL-24 (rhIL-24) was examined by transwell system. Immunohistochemical detection showed that IL-24 was expressed in the villi and decidual tissue, and distributed in villous column,trophoblasts, stroma and blood vessels. The proliferation of TEV-1 cells was not inhibited by rhIL-24of various concentrations. The examination of invasion in vitro showed that rhIL-24 could inhibit the invasion of TEV-1 cells in a concentration-dependent manner. The results suggested IL-24 could inhibit the invasion of TEV-1 cells. Therefore, IL-24 produced by maternal-fetal interface in human first trimester pregnancy may influence the invasion of trophoblasts and is involved in normal pregnancy.
