Effect of YM155 on Apoptosis and Autophagy of K562 Cells.
- Author:
Yi-Han DING
1
;
Xiao-Dong FAN
1
;
Jing-Jing WU
1
;
Zhi-Kui DENG
1
;
Bin WEI
1
;
Yu-Feng LI
2
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Autophagy; Cell Proliferation; Flow Cytometry; Humans; Imidazoles; K562 Cells; Naphthoquinones
- From: Journal of Experimental Hematology 2015;23(2):375-380
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThis study was purposed to investigate the effect of YM155, a survivin inhibitor, on the apoptosis and autophagy of K562 cells.
METHODSK562 cells were treated with YM155 at different concentration. Cell survival was analyzed by CCK-8 assay, the cell apoptosis was detected by flow cytometry. Survivin, BCL-2 and beclin1 mRNA expressions were determined by RT-PCR. Survivin, BCL-2, caspase-3, PARP and LC-3 protein expressions were assayed by Western blot.
RESULTSYM155 inhibited the proliferation of K562 cells in a time- and dose-dependent manners. With the increasing of YM155 concentration and prolonging of action time, the expression levels of mRNA and protein of survivin and BCL-2 decreased, while the expression levels of caspase-3, PARP, beclin1 and LC-3 increased. Compared with the YM155 group, the protein levels of LC-3 and caspase-3 were lower in YM155 combined with 3-MA group.
CONCLUSIONYM155 can inhibit K562 cell proliferation by inducing apoptosis and autophagy, while autophagy induction effect can enhance its cytotoxic effect.
