Bioactive glass 45S5-silk fibroin membrane supports proliferation and differentiation of human dental pulp stem cells
10.3760/cma.j.issn.1002-0098.2015.12.005
- VernacularTitle:生物活性玻璃-丝素蛋白复合膜支持人牙髓干细胞增殖与分化初探
- Author:
Xiaoshuai LYU
1
;
Zhengmao LI
;
Haiyan WANG
;
Xuechao YANG
Author Information
1. 510140,广州医科大学附属口腔医院数字化口腔医疗中心·广州口腔病研究所·口腔医学重点实验室
- Keywords:
Silk;
Dental pulp;
Stem cells;
Bioactivity glasses
- From:
Chinese Journal of Stomatology
2015;50(12):725-730
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of bioactivity glass 45S5-silk fibroin(BG45S5-SF) membrane on growth, proliferation and differentiation of human dental pulp stem cells(hDPSC), and to provide new ideas and method for the regeneration of pulp-dentine complex.Methods hDPSC seed on pure silk fibroin membrane (protein membrane group) and BG45S5-SF membrane with different concentrations(1 000, 5 000 mg/L, composite membrane group A and B, respectively) were prepared, and the materials were incubated in cell culture fluid for 24 h.No material membrane orifice plate was used as blank control group.Contact angle meter was used to measure surface contact angle of protein membrane and composite membrane group(each group had three repeated holes).Cell proliferation was assessed by cell counting kit-8 on the 4, 7, 14, and 21 days.The state of adhesion and growth of hDPSC on the materials surface was evaluated by scanning electron microscopy and cytoskeleton staining;and alkaline phosphatase (ALP) activity was measured to evaluate the cell differentiation potential.The expression of odontoblastic differentiation-related genes was measured by real-time PCR.Results Surface contact angle of the protein membrane group and composite membrane group A and group B were 89.51°±0.12°, 70.32°±0.07° and 71.31°±0.09° respectively.hDPSC adhered well on each materials surface on the 7, 14, 21 days, ALP activity and differentiation genes of composite membrane group A and B rised more significantly than the blank control group and protein membrane group did (P＜0.05).Dentin matrix protein1(DMP-1), dentin sialoprotein(DSP), ALP, osteoealcin(OC) mRNA expression reached peak on the 14 days in group A, and in group B on the 21 days.Bone sialoprotein(BSP) mRNA expression in both group A and B reached peak on the 21 days.Conclusions BG45S5-SF membrane is able to support the proliferation and showed the potential of odontoblastic differentiation for hDPSC.This finding suggests that BG45S5-SF membrane was a kind of tissue engineering film material with the regeneration potential for pulp-dentine complex.
