Characterization of tissue expression of ginsenoside biosynthetic gene expression in Panax ginseng.
10.19540/j.cnki.cjcmm.20170614.007
- Author:
Juan LIU
1
;
Rui-Feng JI
1
;
Tong CHEN
1
;
Yuan YUAN
1
;
Juan GUO
1
;
Ying-Ping WANG
2
;
Wen-Yuan GAO
3
;
Lu-Qi HUANG
1
Author Information
1. State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
2. Institute of Special Animal and Plant, Chinese Academy of Agricultural Sciences, Changchun 130112, China.
3. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
- Publication Type:Journal Article
- Keywords:
ginsenoside biosynthetic genes;
tissue expression;
ginseng
- From:
China Journal of Chinese Materia Medica
2017;42(13):2453-2459
- CountryChina
- Language:Chinese
-
Abstract:
The study is aimed to characterize the tissue expression of 10 key ginsenoside biosynthetic genes using bioinformatics method and real-time quantitative PCR. Heatmap and cluster analysis of 10 ginsenoside biosynthetic genes were performed in four-year-old Jilin ginseng. Using real-time quantitative PCR, the expression correlation of 10 key genes involved in ginsenoside biosynthesis was analyzed in different organs of four-year-old Jilin ginseng including, tissue culture seedling and adventitious root. Pearson correlation was used to analyze the relation between those 10 key genes involved in ginsenoside biosynthesis. The results showed that β-AS and CYP716A52v2 were expressed highly in root of Jilin ginseng and ginseng culture seedling, which was consistent with Ro distribution. In addition, CYP716A53v2 and CYP716A47 which involved in dammarane type ginsenoside biosynthesis were positively correlated, which revealed that the difference of ginsenoside distribution was caused by transport system.
