Analysis of Cordyceps by multi-column liquid chromatography.
10.1016/j.apsb.2016.10.002
- Author:
Zhengming QIAN
1
;
Shaoping LI
2
Author Information
1. Institute of Chinese Medical Sciences, University of Macau, Macao, China; Key Laboratory of State Administration of Traditional Chinese Medicine, Sunshine Lake Pharma Co., Ltd., Dongguan 523850, China.
2. Institute of Chinese Medical Sciences, University of Macau, Macao, China.
- Publication Type:Journal Article
- Keywords:
Cordyceps;
Macromolecules;
Micromolecules;
Multi-column;
Size exclusion column
- From:
Acta Pharmaceutica Sinica B
2017;7(2):202-207
- CountryChina
- Language:English
-
Abstract:
Cordyceps is a famous traditional Chinese medicine (TCM) that has been used in China for hundreds of years. In the present study a multi-column liquid chromatography (MC-LC) system was developed for the qualitative analysis of macromolecules and micromolecules in Cordyceps. The MC-LC system includes a size exclusion pre-column, a size exclusion column (SEC) and a reversed phase column (RP) which were controlled by column-switching valves. The sample was separated by the size exclusion pre-column into two fractions (macromolecules and micromolecules). These fractions were further separated on SEC and RP columns, respectively. A diode array detector (DAD) and a mass spectrometer (MS) were used to detect the components. This MC-LC method was utilized for analysis of Cordyceps samples. Two macromolecular peaks and 15 micromolecular peaks were found in Cordyceps, and 11 of the micromolecular peaks were identified as adenosine-5'-monophosphate (AMP), phenylalanine, uridine, hypoxanthine, inosine, guanine, guanosine, deoxyadenosine-5'-monophosphate (dAMP), adenosine, adenine and cordycepin (or its isomer). This method is useful for quality control of Cordyceps.
