Simultaneous determination of cordycepin and 2'-deoxyadenosine in Cordyceps genus by online SPE-HPLC.
10.19540/j.cnki.cjcmm.20170223.025
- Author:
Ye TIAN
1
;
Chuan-Xi WANG
2
;
Zheng-Ming QIAN
1
;
Zhou LI
1
;
Miao-Xia ZHOU
1
;
Wan-Yang SUN
2
;
Xin-Sheng YAO
2
;
Wen-Jia LI
1
;
Hao GAO
2
Author Information
1. Key Laboratory of State Administration of Traditional Chinese Medicine, Sunshine Lake Pharma Co., Ltd., Dongguan 523850, China.
2. Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China.
- Publication Type:Journal Article
- Keywords:
2′-deoxyadenosine;
HPLC;
SPE;
cordycepin (3′-deoxyadenosine)
- From:
China Journal of Chinese Materia Medica
2017;42(10):1932-1938
- CountryChina
- Language:Chinese
-
Abstract:
An online SPE-HPLC method for simultaneous determination of cordycepin (3'-deoxyadenosine) and 2'-deoxyadenosine in Cordyceps genus (C. sinensis,C. militaris,Hirsutella sinensis and C. sobolifera) was developed. The samples were enriched on a ZORBAX SB-AQ (4.6 mm×12.5 mm,5 μm) column with isocratic elution by 9% methanol solution. The separation of analytes was performed on a ZORBAX SB-AQ (4.6 mm×150 mm,5 μm) column with gradient elution by 0.1% formic acid solution and methanol (91∶9). The flow rate was 1.0 mL•min⁻¹. Column temperature was 40 ℃ and detection wavelength was 260 nm. This method has been applied for analysis of different Cordyceps genus. The 2'-deoxyadenosine was detected in C. sinensis,Hirsutella sinensis and C. sobolifera. The cordycepin was detected in C. militaris. In summary,the cordycepin chromatographic peak from C. sinensis in some past reports may be the 2'-deoxyadenosine chromatographic peak or the mixture peak of 2'-deoxyadenosine and cordycepin in which 2'-deoxyadenosine content was higher than cordycepin. The developed method is suitable for analysis of cordycepin and 2'-deoxyadenosine in Cordyceps genus.
