A quantitative method for simultaneous assay of seven active ingredients with one marker in Scrophularia ningpoensis root.
10.19540/j.cnki.cjcmm.20170419.008
- Author:
Huan-Huan YU
1
;
Meng ZHONG
1
;
Rui DING
1
;
Ting SONG
1
;
Michiyo YOSHIMITSU
2
;
Xiu-Ying XU
2
;
Yi-Min ZHENG
3
Author Information
1. College of Chemistry and Materials Science, Guangxi Teachers Education University, Nanning 530001, China.
2. Department of Pharmacy, Medicine College of Guangxi University of Science and Technology, Liuzhou 545006, China.
3. State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin 541000, China.
- Publication Type:Journal Article
- Keywords:
Scrophularia ningpoensis root;
iridoid glycosides;
quantitative analysis of multi-components by single-marker (QAMS)
- From:
China Journal of Chinese Materia Medica
2017;42(14):2719-2724
- CountryChina
- Language:Chinese
-
Abstract:
To establish a new quantitative method for simultaneous determination of multi-components in Scrophularia root by using high performance liquid chromatography (HPLC) and validate its feasibilities．Meanwhile,using catalpol as one chemical reference substance to establish the relative correct factors and relative retention values of aucubin,harpagide,acteoside,angoroside C,harpagoside and cinnamic acid．Then using the quantitative analysis of multi-components by single-marker (QAMS)model,the six analytes can be quantitatively determined in Scrophularia root．The method was evaluated by comparison of the quantitative results to external standard method．No significant differences were observed between the quantitative results of the two methods．The obtained RCFs were credible．It is feasible and suitable to evaluate the quality of Scrophularia root.
