Structural analysis of 22 full-length hepatitis B virus genomes isolated from patients with hepatocellular carcinoma.
- Author:
Xu LIN
1
;
Xiao XU
;
Da-li ZHENG
;
Jian-yin LIN
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acids; genetics; Carcinoma, Hepatocellular; virology; Cloning, Molecular; DNA, Viral; genetics; Genome, Viral; Genotype; Hepatitis B Core Antigens; genetics; Hepatitis B Surface Antigens; genetics; Hepatitis B virus; genetics; immunology; isolation & purification; Humans; Liver Neoplasms; virology; Mutation; Phylogeny; Promoter Regions, Genetic; Trans-Activators; genetics
- From: Chinese Journal of Oncology 2004;26(4):213-216
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the characteristics of full-length Hepatitis B Virus (HBV) genomes isolated from patients with hepatocellular carcinoma (HCC).
METHODSThe full-length HBV genomes from the serum of HBsAg positive HCC patients were amplified by PCR, and then sequenced and analyzed its structure.
RESULTSTwenty-two full-length HBV DNAs were obtained from different patients of HCC. Phylogenetic analysis revealed that all HBV strains could be categorized into genotype B or C and serotype adr or adw2. Structural analysis showed that HBV obtained shared meaningful consensus mutations in B/T cell epitopes of surface and core antigens, transactivating domain of X protein and enhancer II/core promoter regions as compared to standard strains.
CONCLUSIONGenotype and gene mutation of HBV may be closely correlated with the carcinogenesis of HBV-related hepatocellular carcinoma.
