Establishment of an in vivo model for duck hepatitis B virus infection using Hubei duckling
10.3760/cma.j.issn.1003-9279.2008.02.011
- VernacularTitle:湖北麻鸭乙型肝炎病毒体内感染模型的建立
- Author:
Quan HU
1
;
Yuan FANG
;
Zheng-Mao ZHANG
;
Xiao-Yong ZHANG
;
Zhen-Hua ZHANG
;
Dong-Liang YANG
Author Information
1. 湖北省武汉市疾病预防控制中心
- Keywords:
Hepatitis B virus,duck;
Models,animal;
Genes,viral
- From:
Chinese Journal of Experimental and Clinical Virology
2008;22(2):113-115
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a standard duck hepatitis B virus (DHBV) animal model using a local Hubei species of duck, Ma Ya, and use it as an in vivo experimental system to study antiviral strategies against hepatitis B. Methods Two-day-old Ma Ya ducklings were experimentally infected via intraperitoneal injection with the DHBV inocula, which was collected from the transfected culture supernatant of 1.5-fold-overlength genome recombinant plasmid. Blood samples were taken twice or thrice a week during post-inoculation for 50 days. Viremia was quantified by serum real-time PCR to show the peak. Anfiviral treatment of the DHBV-infected ducklings was show the viremia peak and liver biopsy specimens were analysed by Southern blotting and in-situ hybridization at the end of antiviral drug treatment. Results The experimental infection rate of 2-day-old ducklings was 87.5 % .Viremia started to be detectable on day 7 and reached a peak on day 11 post-inoculation, followed by a decrease and fluctuations. Four weeks of oral administration of 3TC led to a significant decrease in viremia peak during drug treatment. This effect was not sustained, as a rebound in viremia was observed after drug withdrawal. Similarly,the analysis of liver biopsies at the end of 3TC treatment showed a marked decrease in DHBV DNA. However,after drug withdrawal a rebound of intrahepatic DHBV DNA was observed in duck livers. Conclusion The Hubei duck model with experimental DHBV infection of transfected supernatant is more suitable for the hepadnavirus biologic research due to its stability and practicability.
