Effects of matrine on cgi-100 gene expression and proliferation in K562 cells.
- Author:
Feng-Xia HUANG
1
;
Yan ZHANG
;
Wei-Jia WANG
Author Information
1. Key Laboratory of Clinical Examination and Medical Diagnostics, Department of Clinical Biochemistry, Chongqing Medical University, Chongqing 400016, China.
- Publication Type:Journal Article
- MeSH:
Alkaloids;
pharmacology;
Antineoplastic Agents, Phytogenic;
pharmacology;
Cell Proliferation;
drug effects;
Gene Expression Regulation, Neoplastic;
Genes, Neoplasm;
genetics;
physiology;
Humans;
K562 Cells;
Proto-Oncogene Proteins;
genetics;
metabolism;
Quinolizines;
pharmacology
- From:
Journal of Experimental Hematology
2008;16(3):525-530
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the expression level of the unknown cgi-100 gene in human leukemia K562 cells treated with matrine, and to investigate effect of cgi-100 on proliferation of K562 cells. The expression level of cgi-100 was detected by RT-PCR in K562 cells before and after being treated with matrine; pIRES2-EGFP/cgi-100 eukaryotic expression vector was constructed by DNA recombinant technique and was introduced into K562 cells by liposome-mediated DNA transfection. The cgi-100 gene expression level, growth-curve, and cell cycle of the modified K562-cgi-100 cells were detected by RT-PCR, Trypan blue staining and FCM. Morphological changes were observed under the optical and electron microscopes. The results indicated that the expression level of cgi-100 decreased in K562 cells treated with matrine. Heterochromatin decreased, euchromatin and the proportion of S phase in K562-cgi-100 cells increased, and cell proliferation enhanced. It is concluded that the expression of cgi-100 mRNA decreased in a dose- and time-dependent manner in the K562 cells treated with matrine and over-expression of cgi-100 elevates the proliferation and the immaturity level of K562-cgi-100 cells.
