Losartan's effects on the protein expression of the kidney of spontaneous hypertension rat.
- Author:
Yu-xi DU
1
;
Min YU
;
Jun-zhu CHEN
;
Xing-xiang WANG
;
Shu-ping LI
Author Information
- Publication Type:Journal Article
- MeSH: Angiotensin II Type 1 Receptor Blockers; pharmacology; therapeutic use; Animals; Chromatography, Liquid; Electrophoresis, Gel, Two-Dimensional; methods; Hypertension; drug therapy; metabolism; physiopathology; Kidney; metabolism; physiopathology; Losartan; pharmacology; therapeutic use; Male; Proteome; metabolism; Proteomics; methods; Rats; Rats, Inbred SHR; Tandem Mass Spectrometry
- From: Chinese Journal of Applied Physiology 2008;24(4):468-473
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo analyse the alterations of protein expression in the kidney of spontaneously hypertensive rat with losartan.
METHODSThe proteins of the kidney were isolated by two-dimensional gel electrophoresis. The protein spots with significant changes were selected for further identification by LC-MS/MS.
RESULTSThe number of average protein spots of two groups was 570 +/- 48 and 686 +/- 30 respectively. Compared with the SHR, 13 spots had changed significantly after treated with losartan. There were 5 protein spots detected only in SHR group, while 4 up-regulated and 4 down-regulated protein spots were detected in SHR-L group. These differentially expressed proteins were detected by mass spectrometry. 7 spots were identified. There were Heat shock protein (Hsp), Tubulin alpha-1 chain, Transthyretin precursor, Liver regeneration-related protein LRRG03, Ezrin-radixin-moesin binding phosphoprotein 50, Phosphoglycerate kinase 1 and Anionic trypsin I precursor.
CONCLUSIONThe different protein spots expression may play important roles in Losartan's effective protection to hypertension rats renal tissue.
