Splenectomy attenuates severe thermal trauma-induced intestinal barrier breakdown in rats.
10.1007/s11596-015-1520-4
- Author:
Xiang-dong LIU
1
;
Zhen-yong CHEN
2
,
3
;
Peng YANG
4
;
Wen-guang HUANG
4
;
Chun-fang JIANG
4
Author Information
1. Department of Plastic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, 200233, China. liuer_69@sina.com.
2. Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. xhczy@
3. com.
4. Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
- Publication Type:Journal Article
- Keywords:
burn;
endotoxin;
nuclear factor-κB;
occludin;
splenectomy;
tumor necrosis factor-alpha;
zonula occludens protein-1
- MeSH:
Animals;
Blotting, Western;
Endotoxins;
blood;
Female;
Hot Temperature;
Intestinal Mucosa;
physiopathology;
Male;
NF-kappa B;
blood;
Occludin;
metabolism;
Rats;
Rats, Wistar;
Splenectomy;
Tumor Necrosis Factor-alpha;
blood;
Zonula Occludens-1 Protein;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2015;35(6):868-873
- CountryChina
- Language:English
-
Abstract:
The severe local thermal trauma activates a number of systemic inflammatory mediators, such as TNF-α, NF-κB, resulting in a disruption of gut barrier. The gastrointestinal tight junction (TJ) is highly regulated by membrane-associated proteins including zonula occludens protein-1 (ZO-1) and occludin, which can be modulated by inflammatory cytokines. As splenectomy has been shown to reduce secretion of cytokines, we hypothesized that (1) severe scald injury up-regulates TNF-α and NF-κB, meanwhile down-regulates expression of ZO-1 and occludin, leading to the increased intestinal permeability, and (2) splenectomy can prevent the burn-induced decrease in ZO-1 and occludin expression, resulting in improved intestinal barrier. Wistar rats undergoing a 30% total body surface area (TBSA) thermal trauma were randomized to receive an accessorial splenectomy meanwhile or not. Intestinal injury was assessed by histological morphological analysis, and serum endotoxin levels, TNF-α, NF-κB, ZO-1 and occludin levels were detected by Western blotting in the terminal ileum mucosal tissue. 30% TBSA burn caused a significant increase in serum endotoxin levels, but NF-κB, and TNF-α, and the average intestinal villus height and mucosal thickness were decreased significantly. Burn injury could also markedly decrease the levels of ZO-1 and occludin in terminal ileum mucosal tissue (all P<0.01). Splenectomy at 7th day after burn significantly reversed the burn-induced breakdown of ZO-1 and occludin (all P<0.01). The results of this study suggest that severe thermal injury damages the intestinal mucosal barrier. Splenectomy may provide a therapeutic benefit in restoring burn-induced intestinal barrier by decreasing the release of inflammatory cytokines and recovering TJ proteins.
