Interactions of Human T Cell Immunoglobin Mucins with Apoptotic Cells
10.1007/sl1596-012-0002-1
- Author:
CHEN ZHIZHONG
1
;
QING JILIN
;
HU LIHUA
Author Information
1. Department of Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;Department of Laboratory,the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
- Keywords:
apoptosis;
T cell immunoglobulin mucin gene;
fusion protein;
receptor;
immune response;
pervanadate
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2012;32(1):9-16
- CountryChina
- Language:Chinese
-
Abstract:
T cell immunoglobulin mucin (TIM) family playsa key role in regulating immune responses.In this study,the interactions of human TIM family with apoptotic cells were evaluated in order to provide a foundation for further study on the roles of human TIM genes in apoptosis.Nine kinds of pEGFP-N1 eukaryotic expression vectors containing different lengths of the three members of human TIM genes for the expression of TIM-EGFP and the vectors for the expression of TIM-Fc fusion proteins were constructed.It was found that human TIM proteins could recognize and bind to apoptotic cells directly,but not to viable cells.The interactions of sTIM-1-EGFP,sTIM-3-EGFP and sTIM-4-EGFP with apoptotic cells were blocked by TIM-1-Ig,TIM-3-Ig and TIM-4-Ig fusion proteins respectively.In addition,human TIM proteins mediated the recognition of apoptotic cells and bound to apoptotic cells directly via the IgV domains.In conclusion,the TIM family may play a key role in the regulation of apoptosis.Our data also suggest that human TIM proteins probably serve as novel proteins for the detection of the early cellular apoptosis.
