Simultaneous determination of four active compounds in tiangou capsules by HPLC.
- Author:
Xin WANG
1
,
2
;
Lingjun LI
;
Long DAI
Author Information
- Publication Type:Journal Article
- MeSH: Benzyl Alcohols; chemistry; Capsules; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; Flavonoids; chemistry; Furans; chemistry; Glucosides; chemistry; Lignans; chemistry; Linear Models; Organic Chemicals; analysis; Reproducibility of Results; Time Factors
- From: China Journal of Chinese Materia Medica 2011;36(7):868-870
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for quantitative analysis of gastrodin, baicalin, paeonolum and pinoresinol diglucoside in Tiangou capsules by HPLC.
METHODThe separation was performed on SinoChrom ODS-BP column (4.6 mm x 150 mm, 5 microm). The mobile phase consisted of acetonitrile and 0.2% phosphoric acid aqueous solution with gradient elution program. The flow rate was 1.0 ml x min(-1) and the column temperature was 30 degrees C. UV detection wavelength was set at 230 nm.
RESULTThe linear ranges of gastrodin, baicalin, paeonolum and pinoresinol diglucoside were 0.1168-1.1680, 0.1142-1.1420, 0.0512-0.5120, 0.0556-0.5560 microg, respectively. The average recoveries of gastrodin, baicalin, paeonolum and pinoresinol diglucoside were 100.3% (RSD 0.53%), 99.96% (RSD 1.15%), 99.90% (RSD 1.17%), 99.97% (RSD 1.62%), respectively.
CONCLUSIONThe method is accurate, simple, feasible and reliable, and is available for the quality control of Tiangou capsules.
