Expression of miR-181a in Acute Myeloid Leukaemia and Its Effect on Cell Proliferation.

Yue MA; Mu-Xia YAN; Zi-Yan LUO

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Expression of miR-181a in Acute Myeloid Leukaemia and Its Effect on Cell Proliferation.

Author: Yue MA ¹ ; Mu-Xia YAN ² ; Zi-Yan LUO ^{3
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Author Information

1. Out-patient Department of Pediatrics,Guangzhou Women and Children's Medical Center, Guangzhou 510623, Guangdong Province, China.
2. Department of Hematology and Oncology,Guangzhou Women and Children's Medical Center, Guangzhou 510623, Guangdong Province, China.
3. Department of Hematology and Oncology,Guangzhou Women and Children's Medical Center, Guangzhou 510623, Guangdong Province, China. E-mail: yanwo1990@
4. com.
Publication Type:Journal Article
MeSH: Cell Cycle; Cell Line, Tumor; Cell Proliferation; Gene Expression Regulation, Neoplastic; Humans; Leukemia, Myeloid, Acute; MicroRNAs; Real-Time Polymerase Chain Reaction
From: Journal of Experimental Hematology 2016;24(4):985-989
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the expression of miR-181a in AML cell lines and explore its effect on cell proliferation.
METHODSThe expression of miR-181a in AML cell lines (NB4,HL-60,K562 and MV-4-11) was detected by quantiative polymerase chain reation(qPCR). Moreover, the cell proliferation and cell cycle were evaluated in several cell lines (HL60, NB4 and K562) by using CCK-8 and flow cytometry after the imitative transfection with miR-181a.
RESULTSThe miR-181a expression was significantly increased in most AML cell lines, including NB4,HL-60 and MV-4-11, but decreased in a few AML cell lines(K562), as compared with that in control(P<0.05). Overexpressed miR-181a in the cell lines significantly enhanced the cell proliferation, as well as the cell ratio of S-to and G2-phase by miR-181a imitative transfection in vitro.
CONCLUSIONOverexpression of miR-181a can promote AML cell proliferation. MiR-181a may play an oncogene role in AML, studying the MiR-181a may provide a new method for treatment of AML.