Studies on the core functional region of antimicrobial peptide LL-37 for inhibition of RSV replication
10.3760/cma.j.issn.1003-9279.2011.05.012
- VernacularTitle:抗菌肽LL-37抑制呼吸道合胞病毒复制的核心功能区的研究
- Author:
Man TIAN
1
;
De-Yu ZHAO
;
Hong-Wei WANG
Author Information
1. 南京医科大学附属南京儿童医院
- Keywords:
Antimicrobial cationic peptides;
Respiratory syncytial viruses;
Viral care proteins
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(5):355-357
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the core functional region of antimicrobial peptide LL-37,which inhibites RSV replication and could be developed for theraputic aplication.Methods A panel of 6 partial LL-37 peptides ( referred to as P1 to P6 ) was synthesized according to LL-37 amino acide sequence.Hep-2 cells were infected with RSV,treated with LL-37 or partial peptides respectively.Cells were collected after 24 hours incubation at 37℃,CO2 5％.Total RNA was obtained from the cells.Expression level of RSV N gene was quantified by real-time PCR.Meanwhile enzyme-linked immunosorbent assay (ELISA) was used to quantify the chemokines RANTES,IL-8,MCP1 in the supernatants of Hep-2 cultures after 24 h incubation with or without LL-37 and partial peptide P6.Results N-terminal partial LL-37 peptide (corresponding to residues 1-12 of LL-37 ) had no significant effects on RSV replication (P ＞ 0.05 ).In contrast,C-terminal (corresponding to residues 13-37 ) and a panel of 4 overlapping 22-mer partial peptides (from the peptide incorporating aa 13-34 through that spanning aa 16-37 ) showed significant inhibitory effect on RSV replication to some extent ( P ＜ 0.05 or P ＜ 0.01 ).LL-37 induced significant expression of chemokine RANTES,IL-8 and MCP-1 in Hep-2 cells.In contrast,partial peptide P6 had no significant effect on expression of the chemokines in Hep-2 cells.Conclusion The LL-37 C-terminal 22-mer partial peptide P6 was putative core functional region for inhibition of RSV replication.The partial peptide didn't induce significant expression of chemokine RANTES,IL-8 and MCP-1.
