Improved expression of human rotavirus VP6 antigen in the recombinant adenoviruse by codon optimization

VernacularTitle:密码子优化提高重组腺病毒中人轮状病毒VP6基因表达量的研究
Author: Da-Wei TONG ¹ ; Jing-Dong SONG ; Jian-Wei WANG ; Min WANG
Author Information

1. 中国医学科学院中国协和医科大学北京协和医院
Keywords: Codon; Adenoviridae; Rotavirus
From: Chinese Journal of Experimental and Clinical Virology 2011;25(6):420-422
CountryChina
Language:Chinese
Abstract: Objective To increase the recombinant adenovirus vector mediated human rotavirus VP6 gene expression through coden optimization.Methods We have artificially synthesized VP6 gene of group A human rotavirus according to the human biased codon.The modified gene was transfected into 293 cells using adenovirus vector and the gene product,the respective protein was produced.The expression level of optimized gene and wild type gene was detected by Immunofluorescence(IF) and Western Blot.Result A remarkable increase of the expression level of optimized VP6 gene in comparison with the wild-type control.Conclusion The coden optimization indeed help increasing the recombinant adenovirus mediated human rotavirus gene expression,which indicated the potential application of such recombinant adenoviruses in the development of adenoviral-vectored rotavirus vaccines.